A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains

Hexose transporter-deficient yeast strains are valuable testbeds for the study of sugar transport by native and heterologous transporters. In the popular Saccharomyces cerevisiae strain EBY.VW4000, deletion of 21 transporters completely abolished hexose transport. However, repeated use of the LoxP/C...

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Dettagli Bibliografici
Pubblicato in:FEMS Yeast Res
Autori principali: Wijsman, Melanie, Świat, Michał A, Marques, Wesley L, Hettinga, Johanna K, van den Broek, Marcel, de la Torre Cortés, Pilar, Mans, Robert, Pronk, Jack T, Daran, Jean-Marc, Daran-Lapujade, Pascale
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2018
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Research Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6217715/
https://ncbi.nlm.nih.gov/pubmed/30285096
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/femsyr/foy107
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