A toolkit for rapid CRISPR-SpCas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains

Hexose transporter-deficient yeast strains are valuable testbeds for the study of sugar transport by native and heterologous transporters. In the popular Saccharomyces cerevisiae strain EBY.VW4000, deletion of 21 transporters completely abolished hexose transport. However, repeated use of the LoxP/C...

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Detalhes bibliográficos
Publicado no:FEMS Yeast Res
Main Authors: Wijsman, Melanie, Świat, Michał A, Marques, Wesley L, Hettinga, Johanna K, van den Broek, Marcel, de la Torre Cortés, Pilar, Mans, Robert, Pronk, Jack T, Daran, Jean-Marc, Daran-Lapujade, Pascale
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2018
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6217715/
https://ncbi.nlm.nih.gov/pubmed/30285096
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/femsyr/foy107
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