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Time-Resolved Hydroxyl Radical Footprinting of RNA with X-Rays

RNA footprinting by hydroxyl radical cleavage provides ‘snapshots’ of RNA tertiary structure or protein interactions that bury the RNA backbone. Generation of hydroxyl radicals with a high-flux synchrotron X-ray beam provides analysis on a short timescale (5 ms to 100 ms), which enables the structur...

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Detalhes bibliográficos
Publicado no:Curr Protoc Nucleic Acid Chem
Main Authors: Hao, Yumeng, Hulscher, Ryan, Rappé, Mollie C., Adilakshmi, Tadepalli, Woodson, Sarah A., Bohon, Jen, Gupta, Sayan
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6057793/
https://ncbi.nlm.nih.gov/pubmed/29927103
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpnc.52
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