Hydroxyl radical footprinting in vivo: mapping macromolecular structures with synchrotron radiation

We used a high flux synchrotron X-ray beam to map the structure of 16S rRNA and RNase P in viable bacteria in situ. A 300 ms exposure to the X-ray beam was sufficient for optimal cleavage of the phosphodiester backbone. The in vivo footprints of the 16S rRNA in frozen cells were similar to those obt...

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Bibliographic Details
Main Authors: Adilakshmi, Tadepalli, Lease, Richard A., Woodson, Sarah A.
Format: Artigo
Language:Inglês
Published: Oxford University Press 2006
Subjects:
Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1458516/
https://ncbi.nlm.nih.gov/pubmed/16682443
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkl291
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