Probing the Structure of Ribosome Assembly Intermediates In vivo using DMS and Hydroxyl Radical Footprinting

The assembly of the E. coli ribosome has been widely studied and characterized in vitro. Despite this, ribosome biogenesis in living cells is only partly understood because assembly is coupled with transcription, modification and processing of the pre-ribosomal RNA. We present a method for footprint...

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Detalhes bibliográficos
Publicado no:Methods
Main Authors: Hulscher, Ryan M., Bohon, Jen, Rappé, Mollie C., Gupta, Sayan, D’Mello, Rhijuta, Sullivan, Michael, Ralston, Corie Y., Chance, Mark R., Woodson, Sarah A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4921310/
https://ncbi.nlm.nih.gov/pubmed/27016143
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2016.03.012
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