Probing the Structure of Ribosome Assembly Intermediates In vivo using DMS and Hydroxyl Radical Footprinting

The assembly of the E. coli ribosome has been widely studied and characterized in vitro. Despite this, ribosome biogenesis in living cells is only partly understood because assembly is coupled with transcription, modification and processing of the pre-ribosomal RNA. We present a method for footprint...

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Pubblicato in:Methods
Autori principali: Hulscher, Ryan M., Bohon, Jen, Rappé, Mollie C., Gupta, Sayan, D’Mello, Rhijuta, Sullivan, Michael, Ralston, Corie Y., Chance, Mark R., Woodson, Sarah A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2016
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4921310/
https://ncbi.nlm.nih.gov/pubmed/27016143
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2016.03.012
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