Efficient CRISPR‐based genome editing using tandem guide RNAs and editable surrogate reporters

Cleavage efficiency plays a key role in clustered regularly interspaced short palindromic repeat (CRISPR)‐based gene editing, particularly when the given guide RNA exhibits low cleavage activity. Here, we describe the packaging of tandem guide RNAs and single‐strand annealing‐based surrogate reporte...

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Udgivet i:FEBS Open Bio
Main Authors: Liu, Wuqing, Li, Shifeng, Zhang, Yunbin, Li, Jinsong, Li, Yiping
Format: Artigo
Sprog:Inglês
Udgivet: John Wiley and Sons Inc. 2018
Fag:
Methods
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6026697/
https://ncbi.nlm.nih.gov/pubmed/29988563
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/2211-5463.12437
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