Optimized retroviral transduction of mouse T cells for in vivo assessment of gene function

Retroviral (RV) expression of genes of interest (GOIs) is an invaluable tool and has formed the foundation of cellular engineering for adoptive cell therapy in cancer and other diseases. However, monitoring of transduced T cells long term (weeks to months) in vivo remains challenging because of the...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Julkaisussa:Nat Protoc
Päätekijät: Kurachi, Makoto, Kurachi, Junko, Chen, Zeyu, Johnson, John, Khan, Omar, Bengsch, Bertram, Stelekati, Erietta, Attanasio, John, McLane, Laura M, Tomura, Michio, Ueha, Satoshi, Wherry, E John
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 2017
Aiheet:
Article
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC6020692/
https://ncbi.nlm.nih.gov/pubmed/28858287
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2017.083
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