Optimized retroviral transduction of mouse T cells for in vivo assessment of gene function

Retroviral (RV) expression of genes of interest (GOIs) is an invaluable tool and has formed the foundation of cellular engineering for adoptive cell therapy in cancer and other diseases. However, monitoring of transduced T cells long term (weeks to months) in vivo remains challenging because of the...

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Detalhes bibliográficos
Publicado no:Nat Protoc
Main Authors: Kurachi, Makoto, Kurachi, Junko, Chen, Zeyu, Johnson, John, Khan, Omar, Bengsch, Bertram, Stelekati, Erietta, Attanasio, John, McLane, Laura M, Tomura, Michio, Ueha, Satoshi, Wherry, E John
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6020692/
https://ncbi.nlm.nih.gov/pubmed/28858287
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2017.083
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