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Guanine Nucleotide Exchange Assay Using Fluorescent MANT-GDP
GTPases are molecular switches that cycle between the inactive GDP-bound state and the active GTP-bound state. GTPases exchange nucleotides either by its intrinsic nucleotide exchange or by interaction with guanine nucleotide exchange factors (GEFs). Monitoring the nucleotide exchange in vitro, toge...
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| Опубликовано в: : | Bio Protoc |
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| Главные авторы: | , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Bio-Protocol
2018
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6017992/ https://ncbi.nlm.nih.gov/pubmed/29951569 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2795 |
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