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A Mant-GDP Dissociation Assay to Compare the Guanine Nucleotide Binding Preference of Small GTPases

Small GTPases are cellular switches that are switched on when bound to GTP and switched off when bound to GDP. Different small GTPase proteins or those with mutations may bind to GTP or GDP with different relative affinities. However, small GTPases generally have very high affinities for guanine nuc...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Bio Protoc
Main Authors: Tan, Yuping, Sun, Qingxiang
Format: Artigo
Jezik:Inglês
Izdano: Bio-Protocol 2021
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7953247/
https://ncbi.nlm.nih.gov/pubmed/33732775
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3886
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