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A Mant-GDP Dissociation Assay to Compare the Guanine Nucleotide Binding Preference of Small GTPases

Small GTPases are cellular switches that are switched on when bound to GTP and switched off when bound to GDP. Different small GTPase proteins or those with mutations may bind to GTP or GDP with different relative affinities. However, small GTPases generally have very high affinities for guanine nuc...

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Publicado en:Bio Protoc
Autores principales: Tan, Yuping, Sun, Qingxiang
Formato: Artigo
Lenguaje:Inglês
Publicado: Bio-Protocol 2021
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7953247/
https://ncbi.nlm.nih.gov/pubmed/33732775
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3886
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