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A Mant-GDP Dissociation Assay to Compare the Guanine Nucleotide Binding Preference of Small GTPases

Small GTPases are cellular switches that are switched on when bound to GTP and switched off when bound to GDP. Different small GTPase proteins or those with mutations may bind to GTP or GDP with different relative affinities. However, small GTPases generally have very high affinities for guanine nuc...

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Detalles Bibliográficos
Publicado en:Bio Protoc
Main Authors: Tan, Yuping, Sun, Qingxiang
Formato: Artigo
Idioma:Inglês
Publicado: Bio-Protocol 2021
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7953247/
https://ncbi.nlm.nih.gov/pubmed/33732775
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3886
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