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Guanine Nucleotide Exchange Assay Using Fluorescent MANT-GDP

GTPases are molecular switches that cycle between the inactive GDP-bound state and the active GTP-bound state. GTPases exchange nucleotides either by its intrinsic nucleotide exchange or by interaction with guanine nucleotide exchange factors (GEFs). Monitoring the nucleotide exchange in vitro, toge...

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Bibliografiske detaljer
Udgivet i:Bio Protoc
Main Authors: Kanie, Tomoharu, Jackson, Peter K.
Format: Artigo
Sprog:Inglês
Udgivet: Bio-Protocol 2018
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6017992/
https://ncbi.nlm.nih.gov/pubmed/29951569
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2795
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