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Guanine Nucleotide Exchange Assay Using Fluorescent MANT-GDP

GTPases are molecular switches that cycle between the inactive GDP-bound state and the active GTP-bound state. GTPases exchange nucleotides either by its intrinsic nucleotide exchange or by interaction with guanine nucleotide exchange factors (GEFs). Monitoring the nucleotide exchange in vitro, toge...

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Bibliografische gegevens
Gepubliceerd in:Bio Protoc
Hoofdauteurs: Kanie, Tomoharu, Jackson, Peter K.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Bio-Protocol 2018
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6017992/
https://ncbi.nlm.nih.gov/pubmed/29951569
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2795
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