Optimal 3D single-molecule localization in real time using experimental point spread functions

We present a real-time fitter for 3D single-molecule localization microscopy using experimental point spread functions (PSFs) that achieves optimal 3D resolution on any microscope and is compatible with any PSF engineering approach. This allowed us to image cellular structures with a 3D resolution u...

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Bibliografske podrobnosti
izdano v:Nat Methods
Main Authors: Li, Yiming, Mund, Markus, Hoess, Philipp, Deschamps, Joran, Matti, Ulf, Nijmeijer, Bianca, Sabinina, Vilma Jimenez, Ellenberg, Jan, Schoen, Ingmar, Ries, Jonas
Format: Artigo
Jezik:Inglês
Izdano: 2018
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Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6009849/
https://ncbi.nlm.nih.gov/pubmed/29630062
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4661
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