Optimal 3D single-molecule localization in real time using experimental point spread functions

We present a real-time fitter for 3D single-molecule localization microscopy using experimental point spread functions (PSFs) that achieves optimal 3D resolution on any microscope and is compatible with any PSF engineering approach. This allowed us to image cellular structures with a 3D resolution u...

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Detalhes bibliográficos
Publicado no:Nat Methods
Main Authors: Li, Yiming, Mund, Markus, Hoess, Philipp, Deschamps, Joran, Matti, Ulf, Nijmeijer, Bianca, Sabinina, Vilma Jimenez, Ellenberg, Jan, Schoen, Ingmar, Ries, Jonas
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6009849/
https://ncbi.nlm.nih.gov/pubmed/29630062
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4661
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