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Optimizing imaging speed and excitation intensity for single molecule localization microscopy

High laser powers are common practice in single molecule localization microscopy (SMLM) to speed up data acquisition. Here, we systematically quantified how excitation intensity influences localization precision and labeling density, the two main factors determining data quality. We found a strong t...

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Vydáno v:Nat Methods
Hlavní autoři: Diekmann, Robin, Kahnwald, Maurice, Schoenit, Andreas, Deschamps, Joran, Matti, Ulf, Ries, Jonas
Médium: Artigo
Jazyk:Inglês
Vydáno: 2020
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7610360/
https://ncbi.nlm.nih.gov/pubmed/32807954
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41592-020-0918-5
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