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Depth-dependent PSF calibration and aberration correction for 3D single-molecule localization

Three-dimensional single molecule localization microscopy relies on the fitting of the individual molecules with a point spread function (PSF) model. The reconstructed images often show local squeezing or expansion in z. A common cause is depth-induced aberrations in conjunction with an imperfect PS...

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Detalhes bibliográficos
Publicado no:Biomed Opt Express
Main Authors: Li, Yiming, Wu, Yu-Le, Hoess, Philipp, Mund, Markus, Ries, Jonas
Formato: Artigo
Idioma:Inglês
Publicado em: Optical Society of America 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6583355/
https://ncbi.nlm.nih.gov/pubmed/31259045
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.10.002708
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