In vitro reconstitution of sortase-catalyzed pilus polymerization reveals structural elements involved in pilin cross-linking

Covalently cross-linked pilus polymers displayed on the cell surface of Gram-positive bacteria are assembled by class C sortase enzymes. These pilus-specific transpeptidases located on the bacterial membrane catalyze a two-step protein ligation reaction, first cleaving the LPXTG motif of one pilin p...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Chang, Chungyu, Amer, Brendan R., Osipiuk, Jerzy, McConnell, Scott A., Huang, I-Hsiu, Hsieh, Van, Fu, Janine, Nguyen, Hong H., Muroski, John, Flores, Erika, Ogorzalek Loo, Rachel R., Loo, Joseph A., Putkey, John A., Joachimiak, Andrzej, Das, Asis, Clubb, Robert T., Ton-That, Hung
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2018
Assuntos:
PNAS Plus
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6004493/
https://ncbi.nlm.nih.gov/pubmed/29844180
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1800954115
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