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Cas4 nucleases define the PAM, length, and orientation of DNA fragments integrated at CRISPR loci

To achieve adaptive and heritable immunity against viruses and other mobile genetic elements, CRISPR-Cas systems must capture and store short DNA fragments (spacers) from these foreign elements into host genomic CRISPR arrays. This process is catalyzed by conserved Cas1/Cas2 integration complexes, b...

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Bibliographische Detailangaben
Veröffentlicht in:Mol Cell
Hauptverfasser: Shiimori, Masami, Garrett, Sandra C., Graveley, Brenton R., Terns, Michael P.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2018
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5994930/
https://ncbi.nlm.nih.gov/pubmed/29883605
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2018.05.002
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