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CRISPR repeat sequences and relative spacing specify DNA integration by Pyrococcus furiosus Cas1 and Cas2
Acquiring foreign spacer DNA into the CRISPR locus is an essential primary step of the CRISPR–Cas pathway in prokaryotes for developing host immunity to mobile genetic elements. Here, we investigate spacer integration in vitro using proteins from Pyrococcus furiosus and demonstrate that Cas1 and Cas...
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| Vydáno v: | Nucleic Acids Res |
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| Hlavní autoři: | , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Oxford University Press
2019
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6698737/ https://ncbi.nlm.nih.gov/pubmed/31219587 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkz548 |
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