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Kinetics and Fidelity of the Repair of Cas9-Induced Double-Strand DNA Breaks

The RNA-guided DNA endonuclease Cas9 is a powerful tool for genome editing. Little is known about the kinetics and fidelity of the double-strand break (DSB) repair process that follows a Cas9 cutting event in living cells. Here, we developed a strategy to measure the kinetics of DSB repair for singl...

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Detalhes bibliográficos
Publicado no:Mol Cell
Main Authors: Brinkman, Eva K., Chen, Tao, de Haas, Marcel, Holland, Hanna A., Akhtar, Waseem, van Steensel, Bas
Formato: Artigo
Idioma:Inglês
Publicado em: Cell Press 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5993873/
https://ncbi.nlm.nih.gov/pubmed/29804829
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2018.04.016
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