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Frequent sgRNA-barcode recombination in single-cell perturbation assays

Simultaneously detecting CRISPR-based perturbations and induced transcriptional changes in the same cell is a powerful approach to unraveling genome function. Several lentiviral approaches have been developed, some of which rely on the detection of distally located genetic barcodes as an indirect pr...

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Библиографические подробности
Опубликовано в: :PLoS One
Главные авторы: Xie, Shiqi, Cooley, Anne, Armendariz, Daniel, Zhou, Pei, Hon, Gary C.
Формат: Artigo
Язык:Inglês
Опубликовано: Public Library of Science 2018
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC5991360/
https://ncbi.nlm.nih.gov/pubmed/29874289
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0198635
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