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Real‐time observation of flexible domain movements in CRISPR–Cas9

The CRISPR‐associated protein Cas9 is widely used for genome editing because it cleaves target DNA through the assistance of a single‐guide RNA (sgRNA). Structural studies have revealed the multi‐domain architecture of Cas9 and suggested sequential domain movements of Cas9 upon binding to the sgRNA...

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Podrobná bibliografie
Vydáno v:EMBO J
Hlavní autoři: Osuka, Saki, Isomura, Kazushi, Kajimoto, Shohei, Komori, Tomotaka, Nishimasu, Hiroshi, Shima, Tomohiro, Nureki, Osamu, Uemura, Sotaro
Médium: Artigo
Jazyk:Inglês
Vydáno: John Wiley and Sons Inc. 2018
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5978321/
https://ncbi.nlm.nih.gov/pubmed/29650679
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.15252/embj.201796941
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