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A Fluorescence Polarization-Based Competition Assay for Measuring Interactions between Unlabeled Ubiquitin Chains and UCH37•RPN13
Ubiquitin chains regulate distinct signaling events through cooperative interactions with effector proteins and deubiquitinases. Measuring the strength of these interactions is often challenging; either large amounts of material are required or one of the binding partners must be labeled for detecti...
Gardado en:
| Publicado en: | Anal Biochem |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2018
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5976553/ https://ncbi.nlm.nih.gov/pubmed/29698671 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2018.04.018 |
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