लोड हो रहा है...
Uncoupling of sgRNAs from their associated barcodes during PCR amplification of combinatorial CRISPR screens
Many implementations of pooled screens in mammalian cells rely on linking an element of interest to a barcode, with the latter subsequently quantitated by next generation sequencing. However, substantial uncoupling between these paired elements during lentiviral production has been reported, especia...
में बचाया:
| में प्रकाशित: | PLoS One |
|---|---|
| मुख्य लेखकों: | , , , |
| स्वरूप: | Artigo |
| भाषा: | Inglês |
| प्रकाशित: |
Public Library of Science
2018
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| विषय: | |
| ऑनलाइन पहुंच: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5969736/ https://ncbi.nlm.nih.gov/pubmed/29799876 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0197547 |
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