Inducible CRISPR genome editing platform in naive human embryonic stem cells reveals JARID2 function in self-renewal

To easily edit the genome of naïve human embryonic stem cells (hESC), we introduced a dual cassette encoding an inducible Cas9 into the AAVS1 site of naïve hESC (iCas9). The iCas9 line retained karyotypic stability, expression of pluripotency markers, differentiation potential, and stability in 5iLA...

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Veröffentlicht in:Cell Cycle
Hauptverfasser: Ferreccio, Amy, Mathieu, Julie, Detraux, Damien, Somasundaram, Logeshwaran, Cavanaugh, Christopher, Sopher, Bryce, Fischer, Karin, Bello, Thomas, M. Hussein, Abdiasis, Levy, Shiri, Cook, Savannah, Sidhu, Sonia B., Artoni, Filippo, Palpant, Nathan J., Reinecke, Hans, Wang, Yuliang, Paddison, Patrick, Murry, Charles, Jayadev, Suman, Ware, Carol, Ruohola-Baker, Hannele
Format: Artigo
Sprache:Inglês
Veröffentlicht: Taylor & Francis 2018
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5969550/
https://ncbi.nlm.nih.gov/pubmed/29466914
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/15384101.2018.1442621
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