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Stepwise Conversion of a Binding Protein to a Fluorescent Switch: Application to Thermoanaerobacter tengcongensis Ribose Binding Protein
Alternate frame folding (AFF) is a protein engineering methodology the purpose of which is to convert an ordinary binding protein into a molecular switch. The AFF modification entails duplicating an amino- or carboxy-terminal segment of the protein and appending it to the opposite end of the molecul...
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| 出版年: | Biochemistry |
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| 主要な著者: | , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2013
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5966831/ https://ncbi.nlm.nih.gov/pubmed/23302025 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi301105u |
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