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Stepwise Conversion of a Binding Protein to a Fluorescent Switch: Application to Thermoanaerobacter tengcongensis Ribose Binding Protein

Alternate frame folding (AFF) is a protein engineering methodology the purpose of which is to convert an ordinary binding protein into a molecular switch. The AFF modification entails duplicating an amino- or carboxy-terminal segment of the protein and appending it to the opposite end of the molecul...

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Detalhes bibliográficos
Publicado no:Biochemistry
Main Authors: Ha, Jeung-Hoi, Shinsky, Stephen A., Loh, Stewart N.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5966831/
https://ncbi.nlm.nih.gov/pubmed/23302025
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi301105u
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