Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods

Exogenous pathway optimization and chassis engineering are two crucial methods for heterologous pathway expression. The two methods are normally carried out step-wise and in a trial-and-error manner. Here we report a recombinase-based combinatorial method (termed “SCRaMbLE-in”) to tackle both challe...

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Pubblicato in:Nat Commun
Autori principali: Liu, Wei, Luo, Zhouqing, Wang, Yun, Pham, Nhan T., Tuck, Laura, Pérez-Pi, Irene, Liu, Longying, Shen, Yue, French, Chris, Auer, Manfred, Marles-Wright, Jon, Dai, Junbiao, Cai, Yizhi
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group UK 2018
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5964202/
https://ncbi.nlm.nih.gov/pubmed/29789543
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-018-04254-0
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