RNAi screening of subtracted transcriptomes reveals tumor suppression by taurine-activated GABA(A) receptors involved in volume regulation

To identify coding and non-coding suppressor genes of anchorage-independent proliferation by efficient loss-of-function screening, we have developed a method for enzymatic production of low complexity shRNA libraries from subtracted transcriptomes. We produced and screened two LEGO (Low-complexity b...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Wielders, Camiel L. C., van Nierop, Pim, Vormer, Tinke L., Foijer, Floris, Verheij, Joanne, Lodder, Johannes C., Andersen, Jesper B., Mansvelder, Huibert D., te Riele, Hein
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2018
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5963783/
https://ncbi.nlm.nih.gov/pubmed/29787571
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0196979
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