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Construction of a tri-chromatic reporter cell line for the rapid and simple screening of splice-switching oligonucleotides targeting DMD exon 51 using high content screening

Splice-switching oligonucleotides (SSOs) that can modulate RNA splicing are used for the treatment of many genetic disorders. To enhance the efficacy of modulating splicing, it is important to optimize SSOs with regard to target sites, GC content, melting temperature (T(m) value), chemistries, and l...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Shimo, Takenori, Tachibana, Keisuke, Obika, Satoshi
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5955590/
https://ncbi.nlm.nih.gov/pubmed/29768479
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0197373
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