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Construction of a tri-chromatic reporter cell line for the rapid and simple screening of splice-switching oligonucleotides targeting DMD exon 51 using high content screening

Splice-switching oligonucleotides (SSOs) that can modulate RNA splicing are used for the treatment of many genetic disorders. To enhance the efficacy of modulating splicing, it is important to optimize SSOs with regard to target sites, GC content, melting temperature (T(m) value), chemistries, and l...

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Библиографические подробности
Опубликовано в: :PLoS One
Главные авторы: Shimo, Takenori, Tachibana, Keisuke, Obika, Satoshi
Формат: Artigo
Язык:Inglês
Опубликовано: Public Library of Science 2018
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC5955590/
https://ncbi.nlm.nih.gov/pubmed/29768479
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0197373
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