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An Efficient System for Gene Perturbation in Embryonic Hippocampal Progenitors Using Ex Vivo Electroporation Followed by In Vitro Dissociated Cell Culture

We established an efficient cell culture assay that permits combinatorial genetic perturbations in hippocampal progenitors to examine cell-autonomous mechanisms of fate specification. The procedure begins with ex vivo electroporation of isolated, intact embryonic brains, in a manner similar to in ut...

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Опубликовано в: :J Exp Neurosci
Главные авторы: Muralidharan, Bhavana, D’Souza, Leora, Tole, Shubha
Формат: Artigo
Язык:Inglês
Опубликовано: SAGE Publications 2018
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC5946340/
https://ncbi.nlm.nih.gov/pubmed/29760561
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1177/1179069518767404
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