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Efficient transfection of dissociated mouse chromaffin cells using small-volume electroporation

We have developed an improved procedure for isolating and transfecting a chromaffin cell-enriched population of primary cells from adult mouse adrenal glands. Significantly, the parameters of a novel electroporation transfection technique were optimized to achieve an average transfection efficiency...

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Détails bibliographiques
Publié dans:Cytotechnology
Auteurs principaux: Hoerauf, Widmann W., Cazares, Victor A., Subramani, Arasakumar, Stuenkel, Edward L.
Format: Artigo
Langue:Inglês
Publié: Springer Netherlands 2014
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4371572/
https://ncbi.nlm.nih.gov/pubmed/24549789
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-014-9699-y
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