Explant Culture of the Embryonic Mouse Spinal Cord and Gene Transfer by ex vivo Electroporation

Developing axons change responsiveness to guidance cues during the journey to synapse with target cells. Axon crossing at the ventral midline serves as a model for studying how axons accomplish such a switch in their response. Although primary neuron culture has been a versatile technique for elucid...

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Bibliografiske detaljer
Udgivet i:Bio Protoc
Main Authors: Kinoshita-Kawada, Mariko, Hasegawa, Hiroshi, Hongu, Tsunaki, Yanagi, Shigeru, Kanaho, Yasunori, Masai, Ichiro, Mishima, Takayasu, Chen, Xiaoping, Tsuboi, Yoshio, Rao, Yi, Yuasa-Kawada, Junichi, Wu, Jane Y.
Format: Artigo
Sprog:Inglês
Udgivet: Bio-Protocol 2019
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Methods Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7854209/
https://ncbi.nlm.nih.gov/pubmed/33654869
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3373
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