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Topologically knotted deubiquitinases exhibit unprecedented mechanostability to withstand the proteolysis by an AAA+ protease

More than one thousand knotted protein structures have been identified so far, but the functional roles of these knots remain elusive. It has been postulated that backbone entanglement may provide additional mechanostability. Here, we employed a bacterial proteasome, ClpXP, to mechanically unfold 5(...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Sriramoju, Manoj Kumar, Chen, Yen, Lee, Yun-Tzai Cloud, Hsu, Shang-Te Danny
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5935755/
https://ncbi.nlm.nih.gov/pubmed/29728659
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-25470-0
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