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Entropic stabilization of a deubiquitinase provides conformational plasticity and slow unfolding kinetics beneficial for functioning on the proteasome

Human ubiquitin C-terminal hydrolyase UCH-L5 is a topologically knotted deubiquitinase that is activated upon binding to the proteasome subunit Rpn13. The length of its intrinsically disordered cross-over loop is essential for substrate recognition. Here, we showed that the catalytic domain of UCH-L...

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Publicat a:Sci Rep
Autors principals: Lee, Yun-Tzai Cloud, Chang, Chia-Yun, Chen, Szu-Yu, Pan, Yun-Ru, Ho, Meng-Ru, Hsu, Shang-Te Danny
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group 2017
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5364529/
https://ncbi.nlm.nih.gov/pubmed/28338014
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep45174
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