“CapZyme-Seq” comprehensively defines promoter-sequence determinants for RNA 5′ capping with NAD(+)

Nucleoside-containing metabolites such as NAD(+) can be incorporated as “5′ caps” on RNA by serving as non-canonical initiating nucleotides (NCINs) for transcription initiation by RNA polymerase (RNAP). Here, we report “CapZyme-Seq,” a high-throughput-sequencing method that employs NCIN-decapping en...

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Veröffentlicht in:Mol Cell
Hauptverfasser: Vvedenskaya, Irina O., Bird, Jeremy G., Zhang, Yuanchao, Zhang, Yu, Jiao, Xinfu, Barvík, Ivan, Krásný, Libor, Kiledjian, Megerditch, Taylor, Deanne M., Ebright, Richard H., Nickels, Bryce E.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2018
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5935523/
https://ncbi.nlm.nih.gov/pubmed/29681497
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2018.03.014
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