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“NAD-capQ” detection and quantitation of NAD caps

RNA 5′ cap structures comprising the metabolic effector nicotinamide adenine dinucleotide (NAD) have been identified in diverse organisms. Here we report a simple, two-step procedure to detect and quantitate NAD-capped RNA, termed “NAD-capQ.” By use of NAD-capQ we quantitate NAD-capped RNA levels in...

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Bibliografske podrobnosti
izdano v:RNA
Main Authors: Grudzien-Nogalska, Ewa, Bird, Jeremy G., Nickels, Bryce E., Kiledjian, Megerditch
Format: Artigo
Jezik:Inglês
Izdano: Cold Spring Harbor Laboratory Press 2018
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6140466/
https://ncbi.nlm.nih.gov/pubmed/30045887
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.067686.118
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