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On-chip measurements of protein unfolding from direct observations of micron-scale diffusion

Investigations of protein folding, unfolding and stability are critical for the understanding of the molecular basis of biological structure and function. We describe here a microfluidic approach to probe the unfolding of unlabelled protein molecules in microliter volumes. We achieve this objective...

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Détails bibliographiques
Publié dans:Chem Sci
Auteurs principaux: Zhang, Yuewen, Yates, Emma V., Hong, Liu, Saar, Kadi L., Meisl, Georg, Dobson, Christopher M., Knowles, Tuomas P. J.
Format: Artigo
Langue:Inglês
Publié: Royal Society of Chemistry 2018
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5934698/
https://ncbi.nlm.nih.gov/pubmed/29780480
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c7sc04331g
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