CLICK: one-step generation of conditional knockout mice

BACKGROUND: CRISPR/Cas9 enables the targeting of genes in zygotes; however, efficient approaches to create loxP-flanked (floxed) alleles remain elusive. RESULTS: Here, we show that the electroporation of Cas9, two gRNAs, and long single-stranded DNA (lssDNA) into zygotes, termed CLICK (CRISPR with l...

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Bibliografiske detaljer
Udgivet i:BMC Genomics
Main Authors: Miyasaka, Yoshiki, Uno, Yoshihiro, Yoshimi, Kazuto, Kunihiro, Yayoi, Yoshimura, Takuji, Tanaka, Tomohiro, Ishikubo, Harumi, Hiraoka, Yuichi, Takemoto, Norihiko, Tanaka, Takao, Ooguchi, Yoshihiro, Skehel, Paul, Aida, Tomomi, Takeda, Junji, Mashimo, Tomoji
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2018
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5930688/
https://ncbi.nlm.nih.gov/pubmed/29720086
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12864-018-4713-y
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