Directed Evolution to Engineer Monobody for FRET Biosensor Assembly and Imaging at Live-Cell Surface

Monitoring enzymatic activities at the cell surface is challenging due to the poor efficiency of transport and membrane integration of FRET-based biosensors. Therefore, we developed a hybrid biosensor with separate donor and acceptor that assemble in situ. The directed evolution and sequence-functio...

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Detalhes bibliográficos
Publicado no:Cell Chem Biol
Main Authors: Limsakul, Praopim, Peng, Qin, Wu, Yiqian, Allen, Molly, Liang, Jing, Remacle, Albert G., Lopez, Tyler, Ge, Xin, Kay, Brian, Zhao, Huimin, Strongin, Alex Y., Yang, Xiang-Lei, Lu, Shaoying, Wang, Yingxiao
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5910193/
https://ncbi.nlm.nih.gov/pubmed/29396288
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2018.01.002
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