Partial DNA-guided Cas9 enables genome editing with reduced off-target activity

CRISPR–Cas9 is a versatile RNA-guided genome editing tool. Here we demonstrate that partial replacement of RNA nucleotides with DNA nucleotides in CRISPR RNA (crRNA) enables efficient gene editing in human cells. This strategy of partial DNA replacement retains on-target activity when used with both...

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發表在:Nat Chem Biol
Main Authors: Yin, Hao, Song, Chun-Qing, Suresh, Sneha, Kwan, Suet-Yan, Wu, Qiongqiong, Walsh, Stephen, Ding, Junmei, Bogorad, Roman L, Zhu, Lihua Julie, Wolfe, Scot A, Koteliansky, Victor, Xue, Wen, Langer, Robert, Anderson, Daniel G
格式: Artigo
語言:Inglês
出版: 2018
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Article
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5902734/
https://ncbi.nlm.nih.gov/pubmed/29377001
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchembio.2559
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