Partial DNA-guided Cas9 enables genome editing with reduced off-target activity

CRISPR–Cas9 is a versatile RNA-guided genome editing tool. Here we demonstrate that partial replacement of RNA nucleotides with DNA nucleotides in CRISPR RNA (crRNA) enables efficient gene editing in human cells. This strategy of partial DNA replacement retains on-target activity when used with both...

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Detalhes bibliográficos
Publicado no:Nat Chem Biol
Main Authors: Yin, Hao, Song, Chun-Qing, Suresh, Sneha, Kwan, Suet-Yan, Wu, Qiongqiong, Walsh, Stephen, Ding, Junmei, Bogorad, Roman L, Zhu, Lihua Julie, Wolfe, Scot A, Koteliansky, Victor, Xue, Wen, Langer, Robert, Anderson, Daniel G
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5902734/
https://ncbi.nlm.nih.gov/pubmed/29377001
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchembio.2559
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