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Determination of Rab5 Activity in the Cell by Effector Pull-Down Assay

Rab5 targets to early endosomes and is a master regulator of early endosome fusion and endocytosis in all eukaryotic cells. Like other GTPases, Rab5 functions as a molecular switch by alternating between GTP-bound and GDP-bound forms, with the former being biologically active via interactions with m...

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Detalhes bibliográficos
Publicado no:Methods Mol Biol
Main Authors: Qi, Yaoyao, Liang, Zhimin, Wang, Zonghua, Lu, Guodong, Li, Guangpu
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5901726/
https://ncbi.nlm.nih.gov/pubmed/25800849
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2569-8_22
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