Soluble T-cell receptor design influences functional yield in an E. coli chaperone-assisted expression system

There is a quest for production of soluble protein of high quality for the study of T-cell receptors (TCRs), but expression often results in low yields of functional molecules. In this study, we used an E. coli chaperone-assisted periplasmic production system and compared expression of 4 different s...

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Veröffentlicht in:PLoS One
Hauptverfasser: Gunnarsen, Kristin Støen, Høydahl, Lene Støkken, Neumann, Ralf Stefan, Bjerregaard-Andersen, Kaare, Nilssen, Nicolay Rustad, Sollid, Ludvig Magne, Sandlie, Inger, Løset, Geir Åge
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2018
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5897000/
https://ncbi.nlm.nih.gov/pubmed/29649333
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0195868
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