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Chaperone-assisted thermostability engineering of a soluble T cell receptor using phage display

We here report a novel phage display selection strategy enabling fast and easy selection of thermostabilized proteins. The approach is illustrated with stabilization of an aggregation-prone soluble single chain T cell receptor (scTCR) characteristic of the murine MOPC315 myeloma model. Random mutati...

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Detalhes bibliográficos
Main Authors: Gunnarsen, Kristin S., Kristinsson, Solveig G., Justesen, Sune, Frigstad, Terje, Buus, Søren, Bogen, Bjarne, Sandlie, Inger, Løset, Geir Åge
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3557450/
https://ncbi.nlm.nih.gov/pubmed/23362461
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep01162
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