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Improved efficiency of in situ protein analysis by proximity ligation using UnFold probes

We have redesigned probes for in situ proximity ligation assay (PLA), resulting in more efficient localized detection of target proteins. In situ PLA depends on recognition of target proteins by pairs of antibody-oligonucleotide conjugates (PLA probes), which jointly give rise to DNA circles that te...

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發表在:Sci Rep
Main Authors: Klaesson, Axel, Grannas, Karin, Ebai, Tonge, Heldin, Johan, Koos, Björn, Leino, Mattias, Raykova, Doroteya, Oelrich, Johan, Arngården, Linda, Söderberg, Ola, Landegren, Ulf
格式: Artigo
語言:Inglês
出版: Nature Publishing Group UK 2018
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5876389/
https://ncbi.nlm.nih.gov/pubmed/29599435
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-23582-1
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