Compaction of rolling circle amplification products increases signal integrity and signal-to-noise ratio

Rolling circle amplification (RCA) for generation of distinct fluorescent signals in situ relies upon the self-collapsing properties of single-stranded DNA in commonly used RCA-based methods. By introducing a cross-hybridizing DNA oligonucleotide during rolling circle amplification, we demonstrate t...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Clausson, Carl-Magnus, Arngården, Linda, Ishaq, Omer, Klaesson, Axel, Kühnemund, Malte, Grannas, Karin, Koos, Björn, Qian, Xiaoyan, Ranefall, Petter, Krzywkowski, Tomasz, Brismar, Hjalmar, Nilsson, Mats, Wählby, Carolina, Söderberg, Ola
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2015
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4511876/
https://ncbi.nlm.nih.gov/pubmed/26202090
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep12317
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