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Chimeric padlock and iLock probes for increased efficiency of targeted RNA detection

Many approaches exist to detect RNA using complementary oligonucleotides. DNA ligation-based techniques can improve discrimination of subtle sequence variations, but they have been difficult to implement for direct RNA analysis due to the infidelity and inefficiency of most DNA ligases on RNA. In th...

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Detalhes bibliográficos
Publicado no:RNA
Main Authors: Krzywkowski, Tomasz, Kühnemund, Malte, Nilsson, Mats
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6298565/
https://ncbi.nlm.nih.gov/pubmed/30309880
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.066753.118
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