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Fidelity of RNA templated end-joining by chlorella virus DNA ligase and a novel iLock assay with improved direct RNA detection accuracy
Ligation-based nucleic acid detection methods are primarily limited to DNA, since they exhibit poor performance on RNA. This is attributed to reduced end-joining efficiency and/or fidelity of ligases. Interestingly, chlorella virus DNA ligase (PBCV-1 DNA ligase) has recently been shown to possess hi...
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Publicado no: | Nucleic Acids Res |
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Main Authors: | , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Oxford University Press
2017
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5737725/ https://ncbi.nlm.nih.gov/pubmed/29048593 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx708 |
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